Rapid Construction of a Whole-genome Transposon Insertion Collection for Shewanella oneidensis by Knockout Sudoku

@inproceedings{Baym2016RapidCO,
  title={Rapid Construction of a Whole-genome Transposon Insertion Collection for Shewanella oneidensis by Knockout Sudoku},
  author={Michael Baym and Lev Shaket and Isao A. Anzai and Oluwakemi Abiodun Adesina and Buz Barstow},
  year={2016}
}
Whole-genome knockout collections are invaluable for connecting gene sequence to function, yet traditionally they have needed an extraordinary technical effort to construct. Knockout Sudoku is a new method for directing the construction and purification of a curated whole-genome collection of single-gene disruption mutants generated by transposon mutagenesis. Using a simple combinatorial pooling scheme, a highly oversampled collection of transposon mutants can be condensed into a next… CONTINUE READING