Rapid Construction of Stable Infectious Full-Length cDNA Clone of Papaya Leaf Distortion Mosaic Virus Using In-Fusion Cloning

  title={Rapid Construction of Stable Infectious Full-Length cDNA Clone of Papaya Leaf Distortion Mosaic Virus Using In-Fusion Cloning},
  author={Decai Tuo and W. Shen and Pu Yan and Xiaoying Li and Peng Zhou},
  pages={6241 - 6250}
  • Decai Tuo, W. Shen, +2 authors Peng Zhou
  • Published 2015
  • Biology, Medicine
  • Viruses
  • Papaya leaf distortion mosaic virus (PLDMV) is becoming a threat to papaya and transgenic papaya resistant to the related pathogen, papaya ringspot virus (PRSV). The generation of infectious viral clones is an essential step for reverse-genetics studies of viral gene function and cross-protection. In this study, a sequence- and ligation-independent cloning system, the In-Fusion® Cloning Kit (Clontech, Mountain View, CA, USA), was used to construct intron-less or intron-containing full-length… CONTINUE READING
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