Rapid Chagas diagnosis in clinical settings using a multiparametric assay.

Abstract

The development of an immunoassay for detecting circulating antibodies against Trypanosoma cruzi with a good performance appears to be crucial in clinical settings for the management of Chagas disease. Here we propose a new automated ELISA test performed on serum samples using 2 different T. cruzi antigens (a recombinant protein and a T. cruzi whole extract) placed in parallel in separate solid phases. This automated diagnostic tool allows the simultaneous analysis of a large number of sera, by determining the presence of antibodies against both antigens by a single run test, with high sensitivity and specificity. The simultaneous analysis of the reactivity against the 2 antigens in a biparametric modality reduces the percentage of false-negative sera and allows a more accurate diagnosis. Using this multiparametric approach, we propose an effective algorithm for the first step of Chagas diagnosis by performing a single test, with time and cost savings.

DOI: 10.1016/j.diagmicrobio.2012.12.005

Cite this paper

@article{Pierimarchi2013RapidCD, title={Rapid Chagas diagnosis in clinical settings using a multiparametric assay.}, author={Pasquale Pierimarchi and Laura Cerni and Belkisyol{\'e} Alarc{\'o}n de Noya and Giuseppe Nicotera and Zoraida D{\'i}az-Bello and Andrea Angheben and Daria Scacciatelli and Manuela Zonfrillo and Giuliano Recinelli and Annalucia Serafino}, journal={Diagnostic microbiology and infectious disease}, year={2013}, volume={75 4}, pages={381-9} }