Rapid 'de novo' peptide sequencing by a combination of nanoelectrospray, isotopic labeling and a quadrupole/time-of-flight mass spectrometer.

@article{Shevchenko1997RapidN,
  title={Rapid 'de novo' peptide sequencing by a combination of nanoelectrospray, isotopic labeling and a quadrupole/time-of-flight mass spectrometer.},
  author={Andrej Shevchenko and Igor V. Chernushevich and Werner E Ens and Kenneth G. Standing and Bruce Edward Thomson and Matthias Wilm and Matthias Mann},
  journal={Rapid communications in mass spectrometry : RCM},
  year={1997},
  volume={11 9},
  pages={
          1015-24
        }
}
Protein microanalysis usually involves the sequencing of gel-separated proteins available in very small amounts. While mass spectrometry has become the method of choice for identifying proteins in databases, in almost all laboratories 'de novo' protein sequencing is still performed by Edman degradation. Here we show that a combination of the nanoelectrospray ion source, isotopic end labeling of peptides and a quadrupole/ time-of-flight instrument allows facile read-out of the sequences of… CONTINUE READING
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