Rapid, specific, no-wash, far-red fluorogen activation in subcellular compartments by targeted fluorogen activating proteins.

@article{Telmer2015RapidSN,
  title={Rapid, specific, no-wash, far-red fluorogen activation in subcellular compartments by targeted fluorogen activating proteins.},
  author={Cheryl A. Telmer and Richa Verma and Haibing Teng and Susan K. Andreko and Leann Law and Marcel P Bruchez},
  journal={ACS chemical biology},
  year={2015},
  volume={10 5},
  pages={
          1239-46
        }
}
Live cell imaging requires bright photostable dyes that can target intracellular organelles and proteins with high specificity in a no-wash protocol. Organic dyes possess the desired photochemical properties and can be covalently linked to various protein tags. The currently available fluorogenic dyes are in the green/yellow range where there is high cellular autofluorescence and the near-infrared (NIR) dyes need to be washed out. Protein-mediated activation of far-red fluorogenic dyes has the… CONTINUE READING
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