Rapid, sensitive and simple detection method for koi herpesvirus using loop-mediated isothermal amplification.

@article{Yoshino2009RapidSA,
  title={Rapid, sensitive and simple detection method for koi herpesvirus using loop-mediated isothermal amplification.},
  author={Manabu Yoshino and Hajime Watari and Tadashi Kojima and Masanari Ikedo and Jun Kurita},
  journal={Microbiology and immunology},
  year={2009},
  volume={53 7},
  pages={375-83}
}
New methods were developed for the detection of koi herpesvirus (KHV, CyHV-3) by LAMP, which were compared with the PCR for specificity and sensitivity. We designed two primer sets targeting a specific sequence within the 9/5 PCR amplicon (9/5 LAMP) and the upper region of the SphI-5 PCR amplicon (SphI-5 LAMP), including a sequence highly conserved among the strains. The amplification was monitored in real-time based on the increase in turbidity, with magnesium pyrophosphate as the by-product… CONTINUE READING
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