Rapid, sensitive, microscale determination of phosphate in water and soil.

@article{DAngelo2001RapidSM,
  title={Rapid, sensitive, microscale determination of phosphate in water and soil.},
  author={Elisa M. D'Angelo and Jerry M. Crutchfield and M. M. Vandiviere},
  journal={Journal of environmental quality},
  year={2001},
  volume={30 6},
  pages={2206-9}
}
A rapid and sensitive analysis of inorganic and organic phosphorus (P) is needed to analyze water and soil extracts at submicromolar concentrations. The proposed method, based on the complexation of malachite green with phosphomolybdate under acidic conditions, was adapted to a 96-well microtiter plate format, and was tested for matrix interferences using 15 soils and some common extractants, including water, KCI, CaCl2, NaOH, and HCl. The accuracy of P determination was affected when CaCl2 and… CONTINUE READING
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