Rapid, reliable, and cheap point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP (Cap-iLAMP)

@inproceedings{Bokelmann2020RapidRA,
  title={Rapid, reliable, and cheap point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP (Cap-iLAMP)},
  author={Lukas Bokelmann and Olaf Nickel and Tomislav Maricic and Svante Pāābo and Matthias Meyer and Stephan Borte and Stephan Riesenberg},
  booktitle={medRxiv},
  year={2020}
}
Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can easily be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automation, sophisticated laboratory equipment and trained personnel to achieve throughputs that allow whole communities to be tested on a regular basis. Here we present Cap-iLAMP (capture and… Expand
1 Citations
Rapid, point‐of‐care antigen and molecular‐based tests for diagnosis of SARS‐CoV‐2 infection
TLDR
Assessment of the diagnostic accuracy of point‐of‐care antigen and molecular‐based tests for diagnosis of SARS‐CoV‐2 infection found studies of antigen tests were of a higher methodological quality compared to studies of molecular tests, particularly regarding the risk of bias for participant selection and the index test. Expand

References

SHOWING 1-10 OF 41 REFERENCES
Rapid Molecular Detection of SARS-CoV-2 (COVID-19) Virus RNA Using Colorimetric LAMP
TLDR
A method to identify SARS-CoV-2 (COVID-19) virus RNA from purified RNA or cell lysis using loop-mediated isothermal amplification (LAMP) using a visual, colorimetric detection is reported. Expand
Screening for SARS-CoV-2 infections with colorimetric RT-LAMP and LAMP sequencing
TLDR
This study tested a two-color RT-LAMP protocol using clinical SARS-CoV-2 samples and established a protocol that does not require prior RNA isolation and developed a multiplexed LAMP-sequencing protocol as a validation and backup procedure to double-check the results of visual RT- LAMP assays. Expand
Standard operating procedures for SARS-CoV-2 detection by a clinical diagnostic RT-LAMP assay
TLDR
This work presents a clinically-validated standard operating procedure (SOP) for high-throughput SARS- CoV-2 detection by RT-LAMP in 25 minutes that is robust, reliable, repeatable, sensitive, specific, and inexpensive. Expand
Rapid Detection of Novel Coronavirus (COVID-19) by Reverse Transcription-Loop-Mediated Isothermal Amplification
TLDR
RT-LAMP specifically detected COVID-19 in simulated patient samples and could be used for monitoring of exposed individuals or potentially aid with screening efforts in the field and potential ports of entry. Expand
Rapid Detection of SARS-CoV-2 Using Reverse transcription RT-LAMP method
TLDR
The RT-LAMP assay reported here has the advantages of rapid amplification, simple operation, and easy detection, which is useful for the rapid and reliable clinical diagnosis of SARS-CoV-2. Expand
Rapid Detection of Novel Coronavirus (COVID19) by Reverse Transcription-Loop-Mediated Isothermal Amplification
TLDR
R Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) may allow for faster and cheaper field based testing at point-of-risk and potentially aid with screening efforts in the field and potential ports of entry. Expand
A Rapid and Specific Assay for the Detection of MERS-CoV
TLDR
A nucleic acid visualization technique that combines the reverse transcription loop-mediated isothermal amplification technique and a vertical flow visualization strip (RT-LAMP-VF) to detect the N gene of MERS-CoV exhibits high specificity. Expand
Development and Evaluation of a Simple Assay for Marburg Virus Detection Using a Reverse Transcription-Loop-Mediated Isothermal Amplification Method
TLDR
The results obtained by both agarose gel electrophoresis and naked-eye judgment indicated that the RT-LAMP assay developed in this study is an effective tool for the molecular detection of MARV. Expand
Synergistically enhanced colorimetric molecular detection using smart cup: a case for instrument-free HPV-associated cancer screening
TLDR
A smartphone-based synergistically enhanced colorimetric method for molecular diagnostics in the proposed POC smart cup platform is completely compatible with other nucleic acid biomarkers and has great potential for personalized health monitoring and disease prevention. Expand
Specific detection of reverse transcription-loop-mediated isothermal amplification amplicons for Taura syndrome virus by colorimetric dot-blot hybridization.
TLDR
A field diagnosis system based on isothermal reverse transcription-loop-mediated amplification for shrimp Taura syndrome virus (TSV), placing emphasis on specific and simple detection of the LAMP amplicons, and a one-step guanidinium thiocyanate homogenization method to provide RNA extraction efficiency comparable to that of the TRIzol Reagent for RT-LAMP. Expand
...
1
2
3
4
5
...