Rapid, one step staining procedures for analysis of cellular DNA and protein by single and dual laser flow cytometry.

@article{Crissman1982RapidOS,
  title={Rapid, one step staining procedures for analysis of cellular DNA and protein by single and dual laser flow cytometry.},
  author={Harry A. Crissman and John A. Steinkamp},
  journal={Cytometry},
  year={1982},
  volume={3 2},
  pages={84-90}
}
Detailed, simplified techniques are described for simultaneous staining and analysis of DNA and protein in a number of mammalian cell types. Cell staining involves the addition of appropriate dye mixtures to unfixed or ethanol-fixed cells and subsequent analysis of cell populations in the staining reagents generally within 10 to 20 minutes. The approach is novel in that no centrifugation steps are involved during the staining procedure, thus, eliminating cell clumping and cell loss and making… CONTINUE READING

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