Rapid, efficient isolation of murine gonadotropes and their use in revealing control of follicle-stimulating hormone by paracrine pituitary factors.

@article{Wu2004RapidEI,
  title={Rapid, efficient isolation of murine gonadotropes and their use in revealing control of follicle-stimulating hormone by paracrine pituitary factors.},
  author={Joyce C Wu and Pei Qi Su and Nedal W. Safwat and J Scott Sebastian and William L. Miller},
  journal={Endocrinology},
  year={2004},
  volume={145 12},
  pages={5832-9}
}
FSH and LH are produced only in gonadotropes, which are reported to comprise 3-12% of mammalian pituitaries. Factors made within the pituitary are powerful regulators of FSH and also influence LH expression, but their identities and cellular origins are unknown because it is impossible to isolate and individually analyze different pituitary cell types. In this study FSH-producing gonadotropes were specifically tagged in vivo with a transgenic cell surface antigen (H-2Kk) so they could be… CONTINUE READING