Rapid, automated separation of specific bacteria from lake water and sewage by flow cytometry and cell sorting.

@article{Porter1993RapidAS,
  title={Rapid, automated separation of specific bacteria from lake water and sewage by flow cytometry and cell sorting.},
  author={J F Porter and Christine Edwards and Jessica A. Morgan and Roger W. Pickup},
  journal={Applied and environmental microbiology},
  year={1993},
  volume={59 10},
  pages={3327-33}
}
The use of fluorescence-activated flow cytometric cell sorting to obtain highly enriched populations of viable target bacteria was investigated. Preliminary studies employed mixtures of Staphylococcus aureus and Escherichia coli. Cells of S. aureus, when mixed in different proportions with E. coli, could be selectively recovered at a purity in excess of 90%. This was possible even when S. aureus composed only approximately 0.4% of the total cells. Cell sorting was also tested for the ability to… CONTINUE READING

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