Rapid, Fully Automated Digital Immunoassay for p24 Protein with the Sensitivity of Nucleic Acid Amplification for Detecting Acute HIV Infection.

@article{Cabrera2015RapidFA,
  title={Rapid, Fully Automated Digital Immunoassay for p24 Protein with the Sensitivity of Nucleic Acid Amplification for Detecting Acute HIV Infection.},
  author={Carlos Alberto Greg{\'o}rio Cabrera and Lei Chang and Mars Stone and Michael P. Busch and David Henry Wilson},
  journal={Clinical chemistry},
  year={2015},
  volume={61 11},
  pages={1372-80}
}
BACKGROUND Nucleic acid testing (NAT) has become the standard for high sensitivity in detecting low levels of virus. However, adoption of NAT can be cost prohibitive in low-resource settings where access to extreme sensitivity could be clinically advantageous for early detection of infection. We report development and preliminary validation of a simple, low-cost, fully automated digital p24 antigen immunoassay with the sensitivity of quantitative NAT viral load (NAT-VL) methods for detection of… CONTINUE READING