Rapamycin reduces hybridoma cell death and enhances monoclonal antibody production.

@article{Balcarcel2001RapamycinRH,
  title={Rapamycin reduces hybridoma cell death and enhances monoclonal antibody production.},
  author={R. Robert Balcarcel and Gregory Stephanopoulos},
  journal={Biotechnology and bioengineering},
  year={2001},
  volume={76 1},
  pages={1-10}
}
Rapamycin was used as a medium additive to slow the progression of CRL 1606 hybridomas through the cell cycle, under the hypothesis that such a modulation might reduce cell death. Cell cycle distributions for CRL hybridomas in the G1 phase of the cell cycle ranged from 20% to 35% during batch, fed-batch, and continuous culture experiments, independent of culture time, dilution rate, growth rates, or death rates. Rapamycin, an mTOR signaling inhibitor, immunosuppressant, and G1-phase arresting… CONTINUE READING
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