Rap2 as a slowly responding molecular switch in the Rap1 signaling cascade.

@article{Ohba2000Rap2AA,
  title={Rap2 as a slowly responding molecular switch in the Rap1 signaling cascade.},
  author={Yusuke Ohba and Naoki Mochizuki and Koichi Matsuo and Shunichi Yamashita and Michio Nakaya and Yuichi Hashimoto and Makoto Hamaguchi and Tadao Kurata and Kunio Nagashima and Michiyuki Matsuda},
  journal={Molecular and cellular biology},
  year={2000},
  volume={20 16},
  pages={6074-83}
}
Rap2 is a member of the Ras family of GTPases and exhibits 60% identity to Rap1, but the function and regulation of Rap2 remain obscure. We found that, unlike the other Ras family proteins, the GTP-bound active form exceeded 50% of total Rap2 protein in adherent cells. Guanine nucleotide exchange factors (GEFs) for Rap1, C3G, Epac (or cyclic AMP [cAMP]-GEF), CalDAG-GEFI, PDZ-GEF1, and GFR efficiently increased the level of GTP-Rap2 both in 293T cells and in vitro. GTPase-activating proteins… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 38 extracted citations

Similar Papers

Loading similar papers…