• DOI:10.1101/GR.2.1.28
Corpus ID: 32444655

Randomization of genes by PCR mutagenesis.

@article{Cadwell1992RandomizationOG,
  title={Randomization of genes by PCR mutagenesis.},
  author={R. Cadwell and G. F. Joyce},
  journal={PCR methods and applications},
  year={1992},
  volume={2 1},
  pages={
          28-33
        }
}

R. Cadwell, G. F. Joyce

Published 1992

Biology, Medicine

PCR methods and applications

A modified polymerase chain reaction (PCR) was developed to introduce random point mutations into cloned genes. The modifications were made to decrease the fidelity of Taq polymerase during DNA synthesis without significantly decreasing the level of amplification achieved in the PCR. The resulting PCR products can be cloned to produce random mutant libraries or transcribed directly if a T7 promoter is incorporated within the appropriate PCR primer. We used this method to mutagenize the gene… CONTINUE READING

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