Random mutagenesis of a recombinant microbial transglutaminase for the generation of thermostable and heat-sensitive variants.

@article{Marx2008RandomMO,
  title={Random mutagenesis of a recombinant microbial transglutaminase for the generation of thermostable and heat-sensitive variants.},
  author={Christian K. Marx and Thomas C. Hertel and Markus Pietzsch},
  journal={Journal of biotechnology},
  year={2008},
  volume={136 3-4},
  pages={
          156-62
        }
}
  • Christian K. Marx, Thomas C. Hertel, Markus Pietzsch
  • Published in Journal of biotechnology 2008
  • Chemistry, Medicine
  • Recombinant microbial transglutaminase (rMTG), an enzyme useful for the cross-linking or the posttranslational modification of (therapeutic) proteins, was optimized by random mutagenesis for the first time. A screening method was developed which, in addition to state-of-the-art procedures, includes a proteolytic activation step of the expressed soluble pro-enzyme. The library of 5,500 clones was screened for variants with increased thermostability and heat-sensitivity, respectively. Mutant… CONTINUE READING

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