Random mutagenesis by error-prone pol plasmid replication in Escherichia coli.


Directed evolution is an approach that mimics natural evolution in the laboratory with the goal of modifying existing enzymatic activities or of generating new ones. The identification of mutants with desired properties involves the generation of genetic diversity coupled with a functional selection or screen. Genetic diversity can be generated using PCR or… (More)
DOI: 10.1007/978-1-4939-1053-3_3


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