Random mutagenesis and screening of complex glycoproteins: expression of human gonadotropins in Dictyostelium discoideum.

@article{Linskens1999RandomMA,
  title={Random mutagenesis and screening of complex glycoproteins: expression of human gonadotropins in Dictyostelium discoideum.},
  author={Maarten H. K. Linskens and Peter D. J. Grootenhuis and Mieke Blaauw and B Huisman-de Winkel and A van Ravestein and Peter J M Van Haastert and Judith C. Heikoop},
  journal={FASEB journal : official publication of the Federation of American Societies for Experimental Biology},
  year={1999},
  volume={13 6},
  pages={639-45}
}
The soil amoeba Dictyostelium discoideum is a host cell that provides simple genetics in combination with complex protein synthesis. We show that the complex human heterodimeric gonadotropins can be produced and secreted by this organism. Furthermore, both follicle stimulation hormone and choriogonadotropin produced by D. dictyostelium bind to their human receptors and elicit a biological response comparable to the wild-type hormones. We also show that structure-function analysis using random… CONTINUE READING

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