Random insertional mutagenesis of Leptospira interrogans, the agent of leptospirosis, using a mariner transposon.

@article{Bourhy2005RandomIM,
  title={Random insertional mutagenesis of Leptospira interrogans, the agent of leptospirosis, using a mariner transposon.},
  author={Pascale Bourhy and H{\'e}l{\`e}ne Louvel and Isabelle Saint Girons and Mathieu Picardeau},
  journal={Journal of bacteriology},
  year={2005},
  volume={187 9},
  pages={3255-8}
}
The recent availability of the complete genome sequences of Leptospira interrogans, the agent of leptospirosis, has allowed the identification of several putative virulence factors. However, to our knowledge, attempts to carry out gene transfer in pathogenic Leptospira spp. have failed so far. In this study, we show that the Himar1 mariner transposon permits random mutagenesis in the pathogen L. interrogans. We have identified genes that have been interrupted by Himar1 insertion in 35 L… CONTINUE READING
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