Random cloning of genes from mouse chromosome 17.

@article{Kasahara1987RandomCO,
  title={Random cloning of genes from mouse chromosome 17.},
  author={Masanori Kasahara and Felipe Figueroa and J. Klein},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1987},
  volume={84 10},
  pages={3325-8}
}
We describe a method for isolating cosmid clones randomly from mouse chromosome 17. A cosmid library was constructed from the mouse-Chinese hamster cell line R4 4-1 that contains a limited amount of mouse DNA (chromosomes 17 and 18 and some other unidentified material) on a Chinese hamster background. The library was screened with the murine repetitive sequence probe pMBA14, which selectively hybridizes with mouse DNA. The mouse-derived cosmid clones thus identified were individually hybridized… CONTINUE READING