Raman spectroscopy and coherent anti-Stokes Raman scattering imaging: prospective tools for monitoring skeletal cells and skeletal regeneration

@article{Moura2016RamanSA,
  title={Raman spectroscopy and coherent anti-Stokes Raman scattering imaging: prospective tools for monitoring skeletal cells and skeletal regeneration},
  author={Catarina Costa Moura and Rahul S Tare and Richard O. C. Oreffo and Sumeet Mahajan},
  journal={Journal of The Royal Society Interface},
  year={2016},
  volume={13}
}
The use of skeletal stem cells (SSCs) for cell-based therapies is currently one of the most promising areas for skeletal disease treatment and skeletal tissue repair. The ability for controlled modification of SSCs could provide significant therapeutic potential in regenerative medicine, with the prospect to permanently repopulate a host with stem cells and their progeny. Currently, SSC differentiation into the stromal lineages of bone, fat and cartilage is assessed using different approaches… 

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References

SHOWING 1-10 OF 100 REFERENCES

Label-free assessment of adipose-derived stem cell differentiation using coherent anti-Stokes Raman scattering and multiphoton microscopy

Investigation of the potential of nonlinear optical microscopy as a minimally invasive technology to monitor the differentiation of adipose-derived stem cells into adipocytes and osteoblasts indicates that multimodal microscopy has significant potential as an enabling technology for the label-free investigation of SC differentiation.

Raman spectroscopy and CARS microscopy of stem cells and their derivatives.

Raman spectroscopy has revealed that DNA and RNA levels drop when a stem cell differentiates into other cell types, which is linked to a change in the relative sizes of the nucleus and cytoplasm.

Label-free analysis of cellular biochemistry by Raman spectroscopy and microscopy.

Advanced coherent Raman scattering techniques, such as coherent anti-Stokes Raman scattered techniques, are discussed, which allow for the molecularly specific imaging of cells, tissues, and entire organisms in vitro and in vivo.

Coherent anti-Stokes Raman scattering for label-free biomedical imaging

The major biomedical areas where CARS has been applied such as in evaluating liver disease, progression of atherosclerosis, tumour classification and tracking drug delivery, whilst also assessing the future challenges for clinical translation are discussed.

Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy.

A sensitive technique for vibrational imaging of tissues by combining coherent anti-Stokes Raman scattering (CARS) with video-rate microscopy is developed and CARS imaging and spectroscopy of lipid-rich tissue structures in the skin of a live mouse is demonstrated, with unprecedented contrast at subcellular resolution.

In situ analysis of living embryonic stem cells by coherent anti-stokes Raman microscopy.

Coherent anti-Stokes Raman scattering (CARS) microscopy is shown here to be a sensitive and nondestructive method for identifying mouse ESC based on selective observation of specific molecular vibrations believed to be spectroscopic markers indicating the differentiated vs undifferentiated states of such cells.

Quantitative, label-free characterization of stem cell differentiation at the single-cell level by broadband coherent anti-Stokes Raman scattering microscopy.

The exceptionally high speed of spectral imaging with BCARS allows us to chemically map a large number of cells with high spatial resolution, revealing not only the phenotype of individual cells, but also population heterogeneity in the degree of phenotype commitment.

Detection of Osteogenic Differentiation by Differential Mineralized Matrix Production in Mesenchymal Stromal Cells by Raman Spectroscopy

Raman spectroscopy is an excellent biosensor to detect the extent of maturation level during MSCs-osteoblast differentiation with a non-disruptive, real-time and label free manner.
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