Rainbow trout growth hormone: molecular cloning of cDNA and expression in Escherichia coli.

@article{Agellon1986RainbowTG,
  title={Rainbow trout growth hormone: molecular cloning of cDNA and expression in Escherichia coli.},
  author={L. B. Agellon and Thomas T. Chen},
  journal={DNA},
  year={1986},
  volume={5 6},
  pages={463-71}
}
We have isolated several recombinant clones carrying the complementary DNA (cDNA) sequence of the rainbow trout (rt) Salmo gairdneri growth hormone (GH) mRNA by immunoblot screening using an antiserum to chum salmon (Oncorhyncus keta) GH. The nucleotide sequence of one of the rtGH cDNA clones (pAF51) was determined. The rt cDNA sequence in pAF51 encodes a hybrid polypeptide of 199 amino acid residues containing 9 amino acid residues of the bacterial beta-galactosidase, one residue from the… CONTINUE READING

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