Corpus ID: 23932203

Raffinose metabolism in Escherichia coli K12. Purification and properties of a new alpha-galactosidase specified by a transmissible plasmid.

@article{Schmid1976RaffinoseMI,
  title={Raffinose metabolism in Escherichia coli K12. Purification and properties of a new alpha-galactosidase specified by a transmissible plasmid.},
  author={K. Schmid and R. Schmitt},
  journal={European journal of biochemistry},
  year={1976},
  volume={67 1},
  pages={
          95-104
        }
}
  • K. Schmid, R. Schmitt
  • Published 1976
  • Medicine
  • European journal of biochemistry
  • The utilization by Escherichia coli K12 of raffinose as sole carbon source depends on a new raffinose transport system, an invertase and an alpha-galactosidase specified by the Raf-plasmid D1021. The alpha-galactosidase was purified to homogeneity from a mutant strain with constitutive synthesis of the enzyme. alpha-Galactosidase hydrolyzes p-nitrophenyl-alpha-D-galactoside (Km 0.14 mM), methyl-alpha-D-galactoside (Km 30mM), melibiose (Km 3.2 mM) and raffinose (Km 60 mM). The enzymatic activity… CONTINUE READING
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