Raf kinase activation of adenylyl cyclases: isoform-selective regulation.

  title={Raf kinase activation of adenylyl cyclases: isoform-selective regulation.},
  author={Qingming Ding and Robert Gros and Ian D Gray and Ronald Taussig and Stephen S. G. Ferguson and Ross D Feldman},
  journal={Molecular pharmacology},
  volume={66 4},
Adenylyl cyclases (AC), a family of enzymes that catalyze the synthesis of cyclic AMP, are critical regulators of cellular functions. [] Key Result In human embryonic kidney 293 cells, we now demonstrate that in reconstitution studies, c-Raf kinase can mediate phosphorylation of AC VI. Furthermore, AC VI coimmunoprecipitates with c-Raf. Raf kinase-dependent regulation of adenylyl cyclase VI is dependent on the integrity of Ser750 in the fourth intracellular loop of the enzyme and Ser603/Ser608 in the C1b…

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