Radloimmunoassayfor Digoxinwith a FullyAutomatedContinuous- Flow System

Abstract

We describe a fully automated continuous-flow radioimmunoassay for digoxin with use of a Technicon AutoAnalyzer system. It makes use of antibodies linked to magnetizable particles; a magnet separates the bound and free fractions. The precise dispensing of reagents by the AutoAnalyzer system permits a small incubation volume (about 160 tl) and the reproducible timing enables nonequilibrium conditions to be used. Thus a single sample is assayed in 15 mm, with a sample throughput of 30/h. The standard curve ranges from 0.5 to 8.0 zg/llter and is most precise between 1 and 3 tg/liter, the between-assay coefficient of variation being less than 3%. There isno significant carryover between samples of high and low concentration, and results by the method correlate closely (r = 0.969) with those by an established manual assay in which charcoal separation is used.

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Cite this paper

@inproceedings{Greenwood2004RadloimmunoassayforDA, title={Radloimmunoassayfor Digoxinwith a FullyAutomatedContinuous- Flow System}, author={Helena Greenwood and John Landon and Gordon C. Forrest}, year={2004} }