Radioreceptor assay for 1 alpha,25-dihydroxyvitamin D3.

@article{Brumbaugh1974RadioreceptorAF,
  title={Radioreceptor assay for 1 alpha,25-dihydroxyvitamin D3.},
  author={P. F. Brumbaugh and David Haussler and Rubin Bressler and Mark R. Haussler},
  journal={Science},
  year={1974},
  volume={183 4129},
  pages={1089-91}
}
A competitive protein binding assay with a sensitivity of 80 picograms has been developed for 1alpha,25-dihydroxyvitamin D(3), the hormonal form of vitamin D(3). lalpha,25-Dihydroxyvitamin D(3) displaced tritiated hormone from a cytosol-chromatin receptor preparation isolated from chick small intestine, providing a simple assay for the hormone. The concentration of lalpha, 25-dihydroxyvitamin D(3) in human plasma, as determined by this assay, is approximately 6 nanograms per 100 milliliters; in… CONTINUE READING

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