Radioprotection in vitro and in vivo by minicircle plasmid carrying the human manganese superoxide dismutase transgene.

@article{Zhang2008RadioprotectionIV,
  title={Radioprotection in vitro and in vivo by minicircle plasmid carrying the human manganese superoxide dismutase transgene.},
  author={Xichen Zhang and Michael Wayne Epperly and Mark A. Kay and Zhi-ying Chen and Tracy M Dixon and Darcy Franicola and Benjamin A. Greenberger and Paavani Komanduri and Joel S. Greenberger},
  journal={Human gene therapy},
  year={2008},
  volume={19 8},
  pages={820-6}
}
Manganese superoxide dismutase plasmid liposomes (MnSOD-PL) confer organ-specific in vivo ionizing irradiation protection. To prepare for potential intravenous clinical trials of systemic MnSOD-PL for radioprotection in humans, plasmid and bacterial sequences were removed and a new minicircle construct was tested. Minicircle MnSOD was purified and then cotransfected into 32D cl 3 murine interleukin-3-dependent hematopoietic progenitor cells along with another plasmid carrying the neo gene… CONTINUE READING