Radiometric assays for glycerol, glucose, and glycogen.

  title={Radiometric assays for glycerol, glucose, and glycogen.},
  author={Dana C. Bradley and Harvey R Kaslow},
  journal={Analytical biochemistry},
  volume={180 1},
We have developed radiometric assays for small quantities of glycerol, glucose and glycogen, based on a technique described by Thorner and Paulus (1971, J. Biol. Chem. 246, 3885-3894) for the measurement of glycerokinase activity. In the glycerol assay, glycerol is phosphorylated with [32P]ATP and glycerokinase, residual [32P]ATP is hydrolyzed by heating in acid, and free [32P]phosphate is removed by precipitation with ammonium molybdate and triethylamine. Standard dose-response curves were… CONTINUE READING