Radioimmunological measurements of total LH in sheep pituitary cells.

  title={Radioimmunological measurements of total LH in sheep pituitary cells.},
  author={R. P. McIntosh and J. McIntosh},
  journal={Acta endocrinologica},
  volume={102 1},
Procedures commonly used to extract LH from pituitary cells in order to measure total cell content were compared in four cell preparations. It was shown in 81 samples of cells suspended in 1 mM EDTA or 50 mM NaHCO3 that after freezing and thawing followed by any of a variety of treatments, there were no significant differences in the amounts of LH measured by RIA relative to the arbitrarily chosen reference treatment of vigorous pipetting. The additional treatments were multiple freezing and… Expand
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