Radioimmunoassay of tumor necrosis factor in serum.

  title={Radioimmunoassay of tumor necrosis factor in serum.},
  author={A M Teppo and Carl Peter J Maury},
  journal={Clinical chemistry},
  volume={33 11},
We present a double-antibody radioimmunoassay for determination of the concentration of tumor necrosis factor (TNF) in serum. TNF in serum competes with a fixed amount of 125I-labeled TNF for the binding sites of specific rabbit antibodies. The bound TNF is precipitated with Sepharose-bound anti-rabbit IgG, then centrifuged, and the radioactivity of the pellets is counted. The detection limit of the assay is 7 ng/L (B0-3 SD). Bound radioactivity in the range of 10% to 90% of the B0 counts… CONTINUE READING

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