Radioimmunoassay of soluble and insoluble collagenases in fibrotic liver.

  title={Radioimmunoassay of soluble and insoluble collagenases in fibrotic liver.},
  author={Shinya Takahashi and Kenji Koda},
  journal={The Biochemical journal},
  volume={220 1},
We have postulated that an insufficient active of collagenase relative to increased collagen synthesis may be the cause of the increased collagen accumulation in fibrotic tissues. In the present study, 125I-collagenase and rabbit anti-collagenase immunoglobulin G were used to develop a sensitive radioimmunoassay that detects 0.1 nM (3 ng) of collagenase protein in tissue samples. The assay also can detect collagenase protein that is associated with extracellular-matrix collagen fibrils. Good… CONTINUE READING