Radioimmunoassay of rat leptin: sexual dimorphism reversed from humans.

@article{Landt1998RadioimmunoassayOR,
  title={Radioimmunoassay of rat leptin: sexual dimorphism reversed from humans.},
  author={M. Landt and R. Gingerich and P. Havel and W. Mueller and B. Schoner and J. E. Hale and M. Heiman},
  journal={Clinical chemistry},
  year={1998},
  volume={44 3},
  pages={
          565-70
        }
}
  • M. Landt, R. Gingerich, +4 authors M. Heiman
  • Published 1998
  • Chemistry, Medicine
  • Clinical chemistry
  • Adipose tissue secretes leptin, which interacts with receptors in the hypothalamus. In rodent models of obesity, leptin increases metabolism and decreases food intake, which helps to maintain normal body composition. Accurate and precise methods to quantitate circulating leptin concentrations are needed for physiological studies. We developed an RIA to measure leptin in rat plasma, serum, or adipocyte culture fluids. The working range of the assay, defined by the detection limit and the highest… CONTINUE READING
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    References

    SHOWING 1-10 OF 46 REFERENCES
    Radioimmunoassay of leptin in human plasma.
    • 525
    • PDF
    Evidence for Leptin Binding to Proteins in Serum of Rodents and Humans: Modulation With Obesity
    • 255
    • PDF
    Regulation of plasma leptin in mice: influence of age, high-fat diet, and fasting.
    • 287
    • PDF
    Serum immunoreactive-leptin concentrations in normal-weight and obese humans.
    • 6,056
    Plasma Leptin and Insulin Relationships in Obese and Nonobese Humans
    • 416
    • PDF
    Sexual dimorphism in plasma leptin concentration.
    • 548
    • PDF
    Serum leptin levels in women with anorexia nervosa.
    • 405
    • PDF
    Serum leptin concentrations in human immunodeficiency virus-infected men with low adiposity.
    • 73
    Role of leptin in the neuroendocrine response to fasting
    • 2,885