Radioimmunoassay of follistatin: application for in vitro fertilization procedures.

  title={Radioimmunoassay of follistatin: application for in vitro fertilization procedures.},
  author={Mutsubu Sugawara and L V Depaolo and Akira Nakatani and Sheila J. DiMarzo and Nicholas Ling},
  journal={The Journal of clinical endocrinology and metabolism},
  volume={71 6},
A sensitive radioimmunoassay (RIA) for follistatin was developed by using antisera raised in rabbits against purified porcine ovarian follistatin. The displacement curves generated with human follicular fluid and serum were parallel with the standard curve of porcine follistatin. Using this RIA, we have measured human serum follistatin immunoreactivity in six women undergoing ovarian hyperstimulation for in vitro fertilization (IVF). After treatment with a GnRH agonist and gonadotropin, serum… CONTINUE READING

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