Radioimmunoassay of endothelin in human plasma.

  title={Radioimmunoassay of endothelin in human plasma.},
  author={P H Rasmussen and Inger Plum and Niels Eske Bruun and Harriet Dige-Petersen and Thomas Hedner and Jan Hedner and J\orn Giese},
  journal={Blood pressure},
  volume={1 3},
A specific and sensitive radioimmunoassay (RIA) for determination of endothelin-1 (ET-1) in human plasma has been developed. Antibodies were raised in rabbits using synthetic ET-1 conjugated to thyroglobulin as immunogen. The antibodies obtained were used at a final dilution of 1:300,000 yielding maximum binding of 61.7 +/- 3.0% (mean +/- 1 SD, n = 20) of 125I-ET-1. The ID50 (inhibitory dose 50%) was 4.5 +/- 0.6 fmol/100 microliters (mean +/- 1 SD, n = 20). The sensitivity of the RIA was 0.33… CONTINUE READING