Radioimmunoassay of aspartate aminotransferase isoenzymes in human serum.

  title={Radioimmunoassay of aspartate aminotransferase isoenzymes in human serum.},
  author={Fred Leung and A E Niblock and Arthur R. Henderson},
  journal={Clinical chemistry},
  volume={30 8},
We describe the development of a sensitive, specific radioimmunoassay for the cytoplasmic and mitochondrial isoenzymes of human aspartate aminotransferase (L-aspartate:2-oxoglutarate aminotransferase; EC Isoenzymes from human heart tissue were purified to homogeneity and used to raise high-titer antisera in rabbits. We partly purified the antisera by selective column chromatography. The Bolton-Hunter reagent was used to radioiodinate the isoenzymes. The assay requires 100 microL of… CONTINUE READING


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