Corpus ID: 28252146

Radioimmunoassay for serum myoglobin.

@article{Adams1978RadioimmunoassayFS,
  title={Radioimmunoassay for serum myoglobin.},
  author={E. C. Adams and K. Layman and I. Jaunakais},
  journal={Annals of clinical and laboratory science},
  year={1978},
  volume={8 4},
  pages={
          330-41
        }
}
A column flow-through radioimmune assay for serum myoglobin has been developed. The assay uses antibodies to human or rhesus monkey myoglobin coupled to Sepharose by cyanogen bromide activation and myoglobin labeled by the Chloramine-T method. The labeled myoglobin is stable over two to three half-lives. Serum myoglobin levels were elevated when serum was taken up to 12 hours after myocardial infarction and generally elevated up to 24 hours. A continued or repeated elevation of serum myoglobin… Expand
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References

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TLDR
A radioimmunoassay that will measure both normal and above-normal concentrations of myoglobin in serum is described and the possible relationship between serum myoglobin concentration and muscle mass is investigated. Expand
Radioimmunoassay of myoglobin in human serum. Results in patients with acute myocardial infarction.
TLDR
Radioimmunoassay of serum myoglobin appears to be useful and sensitive test for the early detection of myocardial infarction. Expand
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TLDR
Myoglobin was identified in the serum of 11 of 21 patients after myocardial infarction by a sensitive specific complement fixation technic, and larger amounts of myoglobin, 0.4 mug/ml or greater, were found in patients with severe infarctions, three of four of whom died as a result of this illness. Expand
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To the Editor:— This study was undertaken to determine whether analysis for myoglobin in consecutive urine specimens was sufficiently helpful in the diagnosis of myocardial infarction to displaceExpand
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TLDR
All primate skeletal muscle samples tested were equally potent, while beef, rodent and chicken thigh muscle extracts were poorer cross-reactors, and it is postulated that these differences were due to differences in the myoglobin molecules of these species. Expand
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TLDR
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TLDR
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  • E. C. Adams
  • Chemistry, Medicine
  • Annals of clinical laboratory science
  • 1971
The use of several methods to differentiate myoglobin from hemoglobin has been investigated. The immunochemical methods, particularly those of hemagglutination inhibition and radioimmunoassay, areExpand
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TLDR
Insulin levels in a variety of sera assayed by this column method agree well with results from other more conventional radioimmunoassay methods. Expand
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The loss of immunological reactivity at high specific radioactivities or at high levels of chemical substitution with STAI/sup 127/!iodine is demonstrated. Expand
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