Corpus ID: 28252146

Radioimmunoassay for serum myoglobin.

  title={Radioimmunoassay for serum myoglobin.},
  author={E. C. Adams and K. Layman and I. Jaunakais},
  journal={Annals of clinical and laboratory science},
  volume={8 4},
A column flow-through radioimmune assay for serum myoglobin has been developed. The assay uses antibodies to human or rhesus monkey myoglobin coupled to Sepharose by cyanogen bromide activation and myoglobin labeled by the Chloramine-T method. The labeled myoglobin is stable over two to three half-lives. Serum myoglobin levels were elevated when serum was taken up to 12 hours after myocardial infarction and generally elevated up to 24 hours. A continued or repeated elevation of serum myoglobin… Expand
Qualitative bedside assay of increased human serum myoglobin by sandwich dot-immunogold filtration for the diagnosis of acute myocardial infarction.
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Serum myoglobin in primary and secondary skeletal muscle disorders
Results indicate that serum myoglobin is a useful parameter in the assessment of muscle damage in healthy controls and patients with primary and secondary skeletal muscle disorders. Expand
Serum Chemical Biomarkers of Cardiac Injury for Nonclinical Safety Testing
The current status and utility of both recently developed and traditional biomarkers of cardiac injury in nonclinical testing, and considerations for validation of novel biomarkersof cardiac injury are reviewed. Expand


A radioimmunoassay for human serum myoglobin: method development and normal values.
A radioimmunoassay that will measure both normal and above-normal concentrations of myoglobin in serum is described and the possible relationship between serum myoglobin concentration and muscle mass is investigated. Expand
Radioimmunoassay of myoglobin in human serum. Results in patients with acute myocardial infarction.
Radioimmunoassay of serum myoglobin appears to be useful and sensitive test for the early detection of myocardial infarction. Expand
Myoglobinemia following acute myocardial infarction.
Myoglobin was identified in the serum of 11 of 21 patients after myocardial infarction by a sensitive specific complement fixation technic, and larger amounts of myoglobin, 0.4 mug/ml or greater, were found in patients with severe infarctions, three of four of whom died as a result of this illness. Expand
Urinary myoglobin in myocardial infarction.
To the Editor:— This study was undertaken to determine whether analysis for myoglobin in consecutive urine specimens was sufficiently helpful in the diagnosis of myocardial infarction to displaceExpand
Precipitin reactions of anti-human myoglobin serum with several human and animal muscle extracts.
All primate skeletal muscle samples tested were equally potent, while beef, rodent and chicken thigh muscle extracts were poorer cross-reactors, and it is postulated that these differences were due to differences in the myoglobin molecules of these species. Expand
A rapid, flow-through column radioimmunoassay for human chorionic somatomammotropin.
A rapid, flow-through, column, nonequilibrium radioimmunoassay for human chorionic somatomammotropin (HCS) is described and hormone levels in a variety of pregnancy sera assayed by this column method are closely related to results from another more conventional radioimuneassay method. Expand
Myoglobinuria in myocardial infarction
On the day of admission to the hospital, in those patients with acute myocardial infarction, the urinary myoglobin test was positive in a higher percentage of cases than any single serum enzyme determination. Expand
Differentiation of myoglobin and hemoglobin in biological fluids.
  • E. C. Adams
  • Chemistry, Medicine
  • Annals of clinical laboratory science
  • 1971
The use of several methods to differentiate myoglobin from hemoglobin has been investigated. The immunochemical methods, particularly those of hemagglutination inhibition and radioimmunoassay, areExpand
Rapid, column, flow-through radioimmunoassay for insulin.
Insulin levels in a variety of sera assayed by this column method agree well with results from other more conventional radioimmunoassay methods. Expand
The loss of immunological reactivity at high specific radioactivities or at high levels of chemical substitution with STAI/sup 127/!iodine is demonstrated. Expand