Radioimmunoassay for pancreatic glucagon based on specific derivatisation of the hormone.


A specific glucagon antiserum was generated in rabbits using a derivatisation of the methionine residue in glucagon. A sensitive and specific assay for glucagon has been developed. Interference with the assay system by plasma factors was abolished by both extraction of plasma samples and the production of glucagon-free plasma for each individual. 


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