Radioimmunoassay for acetylcholine in the rat brain.

@article{Kawashima1980RadioimmunoassayFA,
  title={Radioimmunoassay for acetylcholine in the rat brain.},
  author={Koichiro Kawashima and Hiromichi Ishikawa and Mitsukuni Mochizuki},
  journal={Journal of pharmacological methods},
  year={1980},
  volume={3 2},
  pages={
          115-23
        }
}
Specific antiserum against acetylcholine (ACh) was produced in the rabbit immunized with a choline hemiglutarate-bovine serum albumin conjugate. The antiserum significantly cross-reacted with choline carboxylates, such as butyrylcholine, succinylcholine, and carbamylcholine. However, neither choline itself nor choline phosphates, such as phosphatidylcholine and phosphorylcholine, showed any significant cross-reaction. The antiserum was used to develop a radioimmunoassay for ACh. The assay can… CONTINUE READING
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