Radioenzymic measurement of norepinephrine, epinephrine, and dopamine: stability, enzymic activity, and sensitivity.

Abstract

We investigated factors affecting sensitivity and reproducibility of radioenzymic (catechol-O-methyltransferase) measurement of plasma catecholamines. There was no apparent deterioration of catecholamine during storage at -20 degrees C for 240 days. Accuracy and sensitivity of the assay depended mostly on the method of transferase preparation; its preparation by adjusting the pH to 6.8 before fractionation with ammonium sulfate resulted in an unsuitable enzyme with a relatively high background-radioactivity count, particularly for neorepinephrine and dopamine.

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@article{Saar1981RadioenzymicMO, title={Radioenzymic measurement of norepinephrine, epinephrine, and dopamine: stability, enzymic activity, and sensitivity.}, author={Nili Saar and Anthony W. Bachmann and R D Gordon}, journal={Clinical chemistry}, year={1981}, volume={27 4}, pages={626-8} }