Radioassay method of neuraminidase towards N-acetylneuraminosyl hexasaccharides.

  title={Radioassay method of neuraminidase towards N-acetylneuraminosyl hexasaccharides.},
  author={Masamitsu Kuriyama and Fuziko Someya and Tambur{\'u}s Nayara Yamada and Tadashi Miyatake},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  volume={119 1-2},
We devised a sensitive method to assay for neuraminidase activities towards alpha-(2 leads to 3)-N-acetylneuraminosyl hexasaccharide and alpha-(2 leads to 6)-N-acetylneuraminosyl hexasaccharide, which were isolated from the urine of a patient with adult sialidosis with partial deficiency of beta-galactosidase. Standard assay conditions for the determination of these neuraminidase activities were established and the radiolabeled reduced derivatives of these substrates were used. The fibroblast… CONTINUE READING