Radiation-mediated proteolysis of CDT1 by CUL4–ROC1 and CSN complexes constitutes a new checkpoint

  title={Radiation-mediated proteolysis of CDT1 by CUL4–ROC1 and CSN complexes constitutes a new checkpoint},
  author={Lisa Higa and Ivailo S. Mihaylov and Damon P. Banks and J. Zheng and H. Zhang},
  journal={Nature Cell Biology},
  • Lisa Higa, Ivailo S. Mihaylov, +2 authors H. Zhang
  • Published 2003
  • Biology, Medicine
  • Nature Cell Biology
  • Genomic integrity is maintained by checkpoints that guard against undesired replication after DNA damage. Here, we show that CDT1, a licensing factor of the pre-replication complex (preRC), is rapidly proteolysed after UV- or γ-irradiation. The preRC assembles on replication origins at the end of mitosis and during G1 to license DNA for replication in S phase. Once the origin recognition complex (ORC) binds to origins, CDC6 and CDT1 associate with ORC and promote loading of the MCM2-7 proteins… CONTINUE READING
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