Radiation inactivation of the glycoprotein, invertase.

@article{Lowe1982RadiationIO,
  title={Radiation inactivation of the glycoprotein, invertase.},
  author={Mark E. Lowe and Ellis S. Kempner},
  journal={The Journal of biological chemistry},
  year={1982},
  volume={257 21},
  pages={12478-80}
}
The radiation target size for invertase activity has been determined for the Saccharomyces cerevisiae glycoprotein which contains 50% carbohydrate. Identical inactivation curves were observed for the native enzyme as well as samples depleted of carbohydrate by incubation with Endo-beta-N-acetylglucosaminidase H. The functional unit of 120,000 daltons was unaltered by the per cent of oligosaccharide cleaved by the enzyme, or by the presence or absence of the released sugars. The irradiated… CONTINUE READING