Radiation Induced DNA Double-Strand Breaks in Radiology.

@article{Kuefner2015RadiationID,
  title={Radiation Induced DNA Double-Strand Breaks in Radiology.},
  author={Michael A. Kuefner and Michael Brand and C Engert and Siegfried Alexander Schwab and Michael Uder},
  journal={RoFo : Fortschritte auf dem Gebiete der Rontgenstrahlen und der Nuklearmedizin},
  year={2015},
  volume={187 10},
  pages={
          872-8
        }
}
  • M. Kuefner, M. Brand, +2 authors M. Uder
  • Published 2 September 2015
  • Medicine
  • RoFo : Fortschritte auf dem Gebiete der Rontgenstrahlen und der Nuklearmedizin
UNLABELLED Shortly after the discovery of X-rays, their damaging effect on biological tissues was observed. The determination of radiation exposure in diagnostic and interventional radiology is usually based on physical measurements or mathematical algorithms with standardized dose simulations. γ-H2AX immunofluorescence microscopy is a reliable and sensitive method for the quantification of radiation induced DNA double-strand breaks (DSB) in blood lymphocytes. The detectable amount of these DNA… 
Persistent DNA Double-Strand Breaks After Repeated Diagnostic CT Scans in Breast Epithelial Cells and Lymphocytes
TLDR
DNA double-strand break induction and repair was investigated in side-by-side comparison of RT treatment with repeated diagnostic CT scans in human breast epithelial cell lines and lymphoblastoid cells harboring different mutations in known DNA damage repair proteins.
RISK EVALUATION IN THE LOW-DOSE RANGE CT FOR RADIATION-EXPOSED CHILDREN, BASED ON DNA DAMAGE
TLDR
An overview of previously published data is provided and the current state of knowledge is summarized to help draw conclusions about the effects of CT examinations and risk of carcinogenesis.
Evaluating Gamma-H2AX Expression as a Biomarker of DNA Damage after X-ray in Angiography Patients
TLDR
Investigation of phosphorylation of Histone H2AX in the location of double-stranded breaks in peripheral blood lymphocytes as an indication of biological effects of radiation on angiography finds that this technique could play a significant role in monitoring patients.
DNA double-strand breaks of human peripheral blood lymphocyte induced by CT examination of oral and maxillofacial region
TLDR
A CT examination of the head and neck region provides a high enough radiation dose to induce DNA double-strand breaks in cells in the peripheral blood and there was a linear correlation between the formation of DNAdouble- Strand breaks and radiation doses after CT examination.
Quantification of DNA Double Strand Breaks and Oxidation Response in Children and Adults Undergoing Dental CBCT Scan
TLDR
It is indicated that children and adults react differently to IR doses associated with CBCT, and fully understanding these differences could lead to an optimal use of CBCT in different age categories as well as improved radiation protection guidelines.
Radioprotective agents to prevent cellular damage due to ionizing radiation
TLDR
The data suggest that radioprotective agents working through a variety of mechanisms have the potential to decrease free radical damage produced by ionizing radiation.
The effect of prophylactic oral vitamin C use on DNA double-strand breaks after abdominal contrast-enhanced CT: A preliminary study.
TLDR
Oral Vitamin C can significantly reduce the level of DSBs after abdominal contrast-enhanced CT examination and is a simple and effective method to decrease DNA damage.
The effect of abdominal contrast-enhanced CT on DNA double-strand breaks in peripheral blood lymphocytes: an in vitro and in vivo study
TLDR
Abdominal CE-CT significantly increased DSB levels in both in vitro and in vivo experiments, and a positive linear correlation of CT radiation dose with intracellular DSBs levels was observed in the in vitro study.
Enhanced radiation damage caused by iodinated contrast agents during CT examination.
TLDR
The iodinated contrast agent itself can lead to an increase in the level of DSBs as assessed with γH2AX foci formation, and the application of ICA can amplify DNA damage induced by diagnostic x-ray procedures such as whole abdominal CT.
Potential application of γ-H2AX as a biodosimetry tool for radiation triage.
TLDR
Measurement of γ-H2AX foci formed at and around the sites of DNA double-strand breaks is a rapid and sensitive biodosimetry method which does not require culturing and is thus promising for the analysis of a large number of samples.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 39 REFERENCES
In vivo formation and repair of DNA double-strand breaks after computed tomography examinations.
  • M. Löbrich, N. Rief, +4 authors M. Uder
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 2005
TLDR
The data show that the in vivo induction and repair of DSBs can be assessed in individuals exposed to low radiation doses, adding a further dimension to DSB repair studies and providing the opportunity to identify repair-compromised individuals after diagnostic irradiation procedures.
[X-ray-induced DNA double-strand breaks after angiographic examinations of different anatomic regions].
PURPOSE The aim of this study was to investigate DNA double-strand breaks (DSBs) in blood lymphocytes as markers of the biological radiation effects in angiography patients. MATERIALS AND METHODS
Evidence for a lack of DNA double-strand break repair in human cells exposed to very low x-ray doses
  • K. Rothkamm, M. Löbrich
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 2003
TLDR
Evidence is presented that foci of γ-H2AX (a phosphorylated histone), detected by immunofluorescence, are quantitatively the same as DSBs and are capable of quantifying the repair of individual D SBs, allowing the investigation of DSB repair after radiation doses as low as 1 mGy, an improvement by several orders of magnitude over current methods.
Measurement of H2AX Phosphorylation as a Marker of Ionizing Radiation Induced Cell Damage
TLDR
This chapter describes available methods for gammaH2AX detection and discusses its possible use in clinical practice, and focuses on radiation-induced DNA damage and its biological effects.
DNA double-strand breaks as potential indicators for the biological effects of ionising radiation exposure from cardiac CT and conventional coronary angiography: a randomised, controlled study
TLDR
DNA double-strand break assessment may provide a strategy for individualised assessments of radiation exposure andEstimations of the biological effects of ionising radiation may become feasible.
Contrast medium-enhanced radiation damage caused by CT examinations.
TLDR
The highly sensitive gammaH2AX foci assay shows that CM-enhanced radiation damage incurred in peripheral lymphocytes during CT shows that long-term bioeffects of low-dose ionizing radiation from CT examinations, such as cancer, are increased by using CM.
Effect of CT scan protocols on x-ray-induced DNA double-strand breaks in blood lymphocytes of patients undergoing coronary CT angiography
TLDR
γ-H2AX immunofluorescence microscopy allows one to determine dose-related effects on x-ray-induced DSB levels and to consider individual factors which cannot be monitored by physical dose measurements.
DNA Double-Strand Breaks and Their Repair in Blood Lymphocytes of Patients Undergoing Angiographic Procedures
TLDR
&ggr;-H2AX immunofluorescence microscopy is a reliable and sensitive method for measuring the induction and repair of DNA damage caused by ionizing radiation during angiography.
Intravenous Iodinated Contrast Agents Amplify DNA Radiation Damage at CT.
TLDR
The application of iodinated contrast agents during diagnostic x-ray procedures, such as chest CT, leads to a clear increase in the level of radiation-induced DNA damage as assessed with γH2AX foci formation.
Induction and repair of DNA double-strand breaks in blood lymphocytes of patients undergoing 18F-FDG PET/CT examinations
TLDR
PET/CT-induced DSBs can be monitored using γ-H2AX immunofluorescence microscopy and to differentiate between 18F-fluorodeoxyglucose (FDG) and CT-induced DNA lesions.
...
1
2
3
4
...