Radiation Brightening from Virus-Like Particles.

@article{Tsvetkova2019RadiationBF,
  title={Radiation Brightening from Virus-Like Particles.},
  author={Irina B. Tsvetkova and Arathi Anil Sushma and Joseph C.-Y. Wang and William L. Schaich and Bogdan Dragnea},
  journal={ACS nano},
  year={2019}
}
Concentration quenching is a well-known challenge in many fluorescence imaging applications. Here we show that the optical emission from hundreds of chromophores confined onto the surface of a virus particle 28 nm diameter can be recovered under pulsed irradiation. We have found that, as one increases the number of chromophores tightly-bound to the virus surface, fluorescence quenching ensues at first, but when the number of chromophores per particle is nearing the maximum number of surface… 
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  • B. Dragnea
  • OSA Advanced Photonics Congress 2021
  • 2021

References

SHOWING 1-10 OF 52 REFERENCES
An exciton-polariton laser based on biologically produced fluorescent protein
TLDR
The unique molecular structure of eGFP prevents exciton annihilation even at high excitation densities, thus facilitating polariton condensation under conventional nanosecond pumping, and is clearly evidenced by a distinct threshold, an interaction-induced blueshift of the condensate, long-range coherence, and the presence of a second threshold at higher excitation density that is associated with the onset of photon lasing.
Modern fluorescent proteins: from chromophore formation to novel intracellular applications.
TLDR
The current knowledge of blue, green, and red chromophore formation in permanently emitting FPs, photoactivatable FP, and fluorescent timers is reviewed.
Manipulating Excited-State Dynamics of Individual Light-Harvesting Chromophores through Restricted Motions in a Hydrated Nanoscale Protein Cavity.
TLDR
The results presented herein show that fully hydrated nanoscale protein cavities are a promising way to mimic the tight protein pockets found in natural light-harvesting complexes and the interplay between protein, solvent, and chromophores can be used to significantly improve the yield of interchromophore energy transfer and extend the range of excitation transport.
Fluorescent signal amplification of carbocyanine dyes using engineered viral nanoparticles.
TLDR
These viral nanoparticles markedly increased assay sensitivity in a microarray-based genotyping assay for Vibrio cholerae O139, thus demonstrating their applicability for existing DNA microarray protocols.
Core-controlled polymorphism in virus-like particles
TLDR
Using electron microscopy with image reconstruction, the structures of several VLPs obtained from brome mosaic virus capsid proteins and gold nanoparticles were elucidated and found to resemble to three classes of viral particles found in cells, which form three-dimensional crystals under the same conditions as the wild-type virus.
Constructing and exploiting the fluorescent protein paintbox (Nobel Lecture).
TLDR
The best way to create a fluorescent sensor to detect cAMP with the necessary affinity and selectivity inside cells would be to hijack a natural cAMP-binding protein, now more commonly abbreviated PKA.
Nobel lecture: constructing and exploiting the fluorescent protein paintbox.
  • R. Tsien
  • Biology
    Integrative biology : quantitative biosciences from nano to macro
  • 2010
TLDR
The best way to create a fluorescent sensor to detect cAMP with the necessary affinity and selectivity inside cells would be to hijack a natural cAMP-binding protein, now more commonly abbreviated PKA.
Superradiance and Exciton Delocalization in Bacterial Photosynthetic Light-Harvesting Systems
We present temperature-dependent fluorescence quantum yield and lifetime measurements on the LH-1 and LH-2 complexes of Rhodobacter sphaeroides and on the isolated B820 subunit of Rhodospirillum…
Engineering Recombinant Virus-like Nanoparticles from Plants for Cellular Delivery.
TLDR
Plant-based transient expression of the Bluetongue virus structural proteins, VP3 and VP7, are used to obtain high yields of empty and green fluorescent protein (GFP)-encapsidating core-like particles (CLPs) from leaves to highlight the importance of detailed structural analysis of VNPs in validating their molecular organization.
Room-temperature spontaneous superradiance from single diamond nanocrystals
TLDR
The results pave the way towards a systematic study of SR in a well-controlled, solid-state quantum system at room temperature from single, highly luminescent diamond nanocrystals with spatial dimensions much smaller than the wavelength of light.
...
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2
3
4
5
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