Radial and longitudinal diffusion of myoglobin in single living heart and skeletal muscle cells.

@article{Papadopoulos2001RadialAL,
  title={Radial and longitudinal diffusion of myoglobin in single living heart and skeletal muscle cells.},
  author={Simon Papadopoulos and Volker Endeward and B Revesz-Walker and Klaus Jurgens and Gerolf Gros},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2001},
  volume={98 10},
  pages={5904-9}
}
We have used a fluorescence recovery after photobleaching (FRAP) technique to measure radial diffusion of myoglobin and other proteins in single skeletal and cardiac muscle cells. We compare the radial diffusivities, D(r) (i.e., diffusion perpendicular to the long fiber axis), with longitudinal ones, D(l) (i.e., parallel to the long fiber axis), both measured by the same technique, for myoglobin (17 kDa), lactalbumin (14 kDa), and ovalbumin (45 kDa). At 22 degrees C, D(l) for myoglobin is 1.2 x… CONTINUE READING

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The Mathematics of Diffusion (Clarendon, Oxford)

  • J. Crank
  • 1956

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