Rad53 kinase activation-independent replication checkpoint function of the N-terminal forkhead-associated (FHA1) domain.

@article{Pike2004Rad53KA,
  title={Rad53 kinase activation-independent replication checkpoint function of the N-terminal forkhead-associated (FHA1) domain.},
  author={Brietta L. Pike and Nora Tenis and J{\"o}rg Heierhorst},
  journal={The Journal of biological chemistry},
  year={2004},
  volume={279 38},
  pages={39636-44}
}
Saccharomyces cerevisiae Rad53 has crucial functions in many aspects of the cellular response to DNA damage and replication blocks. To coordinate these diverse roles, Rad53 has two forkhead-associated (FHA) phosphothreonine-binding domains in addition to a kinase domain. Here, we show that the conserved N-terminal FHA1 domain is essential for the function of Rad53 to prevent the firing of late replication origins in response to replication blocks. However, the FHA1 domain is not required for… CONTINUE READING