Rad52 associates with RPA and functions with rad55 and rad57 to assemble meiotic recombination complexes.

@article{Gasior1998Rad52AW,
  title={Rad52 associates with RPA and functions with rad55 and rad57 to assemble meiotic recombination complexes.},
  author={S. Gasior and A. K. Wong and Y. Kora and A. Shinohara and D. K. Bishop},
  journal={Genes \& development},
  year={1998},
  volume={12 14},
  pages={
          2208-21
        }
}
We show that the Saccharomyces cerevisiae recombination protein Rad52 and the single-strand DNA-binding protein RPA assemble into cytologically detectable subnuclear complexes (foci) during meiotic recombination. Immunostaining shows extensive colocalization of Rad52 and RPA and more limited colocalization of Rad52 with the strand exchange protein Rad51. Rad52 and RPA foci are distinct from those formed by Rad51, and its meiosis-specific relative Dmc1, in that they are also detected in meiosis… Expand
Remodeling of the Rad51 DNA Strand-Exchange Protein by the Srs2 Helicase
TLDR
It is shown that Srs2 indeed disrupts Rad51-containing complexes from chromosomes during meiosis, and suggests that a precise level of SRS2, in the form of the Srs1 translocase, is required to appropriately regulate the Rad51 nucleoprotein filament dynamics during meiotic. Expand
In vivo roles of Rad52, Rad54, and Rad55 proteins in Rad51-mediated recombination.
TLDR
Ch chromatin immunoprecipitation is used to monitor the in vivo association of Saccharomyces cerevisiae Rad51p with both the cleaved MATa locus and the HML alpha donor, providing evidence of the time needed for the Rad51 filament to search the genome for a homologous sequence. Expand
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TLDR
Results suggest that RAD51AP1 also serves an important role in meiotic homologous recombination, and provide evidence that functional cooperation is dependent on complex formation between DMC1 and RAD51 AP1 and that distinct epitopes in RAD 51AP1 mediate interactions with RAD51 and D MC1. Expand
RAD54 is essential for RAD51-mediated repair of meiotic DSB in Arabidopsis
TLDR
It is shown here that RAD54 is required for repair of meiotic double strand breaks by RAD51 in the plant Arabidopsis thaliana, and this function is downstream of the meiotic role of RAD 51 in supporting the activity of DMC1, providing new insights into the regulation of the central step of homologous recombination in plants and very probably also other multicellular eukaryotes. Expand
RAD54 is essential for RAD51-mediated repair of meiotic DSB in Arabidopsis
An essential component of the homologous recombination machinery in eukaryotes, the RAD54 protein is a member of the SWI2/SNF2 family of helicases with dsDNA-dependent ATPase, DNA translocase, DNAExpand
The Rad51 Paralog Rad51B Promotes Homologous Recombinational Repair
TLDR
Findings show that Rad51B is important for repairing various types of DNA lesions and maintaining chromosome integrity, and promotes the assembly of Rad51 nucleoprotein filaments during HRR. Expand
Role of the Saccharomyces cerevisiae Rad51 Paralogs in Sister Chromatid Recombination
TLDR
It is suggested that most spontaneous SCR initiates from single-stranded gaps formed at stalled replication forks rather than DSBs, and that Rad55 and Rad57 have different roles in the recombinational repair of stalled replication fork repair compared with DSB repair. Expand
The mitotic DNA damage checkpoint proteins Rad17 and Rad24 are required for repair of double-strand breaks during meiosis in yeast.
We show here that deletion of the DNA damage checkpoint genes RAD17 and RAD24 in Saccharomyces cerevisiae delays repair of meiotic double-strand breaks (DSBs) and results in an altered ratio ofExpand
Coprinus cinereus rad50 Mutants Reveal an Essential Structural Role for Rad50 in Axial Element and Synaptonemal Complex Formation, Homolog Pairing and Meiotic Recombination
TLDR
It is thought it likely that arrest in both mre11-1 and the collection of rad50 mutants is the result of unrepaired or improperly processed DSBs in the genome and that Rad50 and Mre11 are dispensable in C. cinereus for DSB formation, but required for appropriate DSB processing. Expand
Genetic evidence suggests that Spata22 is required for the maintenance of Rad51 foci in mammalian meiosis
TLDR
It is shown that a protein with unknown function, Spata22, colocalises with Rpa in meiotic nodules in rat spermatocytes, and a possible model of presynaptic filament formation in mammalian meiosis, which involves Rpa and Spata 22 is proposed. Expand
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By several independent criteria, RAD55- and RAD57-encoded products are shown here to exist as a stable heterodimer, providing evidence for a role of the Rad55-Rad57heterodimer in overcoming the inhibitory effect of RPA. Expand
Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein
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It is suggested that the Rad51 protein, probably together with Rad52 protein, is involved in a step to convert DSBs to the next intermediate in recombination. Expand
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Rad52 functions as a co-factor for the Rad51 recombinase, acting specifically to overcome the apparent competition by RPA for binding to single-stranded DNA. Expand
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TLDR
It is shown that Rad52 protein stimulates Rad51 reactions and that binding to Rad51 is necessary for this stimulatory effect, and that this binding is crucial in recombination and that it facilitates the formation of Rad51 nucleoprotein filaments. Expand
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TLDR
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TLDR
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TLDR
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TLDR
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Rad52 protein stimulates DNA strand exchange by Rad51 and replication protein A
TLDR
It is found that Rad52 protein stimulates DNA strand exchange by targeting Rad51 protein to a complex of replication protein A (RPA) with single-stranded DNA, implying that specific protein–protein interactions between Rad52protein, Rad51protein and RPA are required. Expand
RecA homologs Dmc1 and Rad51 interact to form multiple nuclear complexes prior to meiotic chromosome synapsis
TLDR
Dmc1 and Rad51, yeast homologs of the E. coli RecA protein, are shown by immunostaining to localize to as many as 64 sites within spread meiotic nuclei, suggesting they represent recombination intermediates. Expand
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