Rad51‐deficient vertebrate cells accumulate chromosomal breaks prior to cell death

@article{Sonoda1998Rad51deficientVC,
  title={Rad51‐deficient vertebrate cells accumulate chromosomal breaks prior to cell death},
  author={E. Sonoda and M. Sasaki and J. Buerstedde and O. Bezzubova and A. Shinohara and H. Ogawa and M. Takata and Y. Yamaguchi-Iwai and S. Takeda},
  journal={The EMBO Journal},
  year={1998},
  volume={17}
}
Yeast rad51 mutants are viable, but extremely sensitive to γ‐rays due to defective repair of double‐strand breaks. In contrast, disruption of the murine RAD51 homologue is lethal, indicating an essential role of Rad51 in vertebrate cells. We generated clones of the chicken B lymphocyte line DT40 carrying a human RAD51 transgene under the control of a repressible promoter and subsequently disrupted the endogenous RAD51 loci. Upon inhibition of the RAD51 transgene, Rad51− cells accumulated in the… Expand
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