Rad18 regulates DNA polymerase kappa and is required for recovery from S-phase checkpoint-mediated arrest.

@article{Bi2006Rad18RD,
  title={Rad18 regulates DNA polymerase kappa and is required for recovery from S-phase checkpoint-mediated arrest.},
  author={Xiaohui Bi and Laura R. Barkley and D. McLaughlan Slater and Satoshi Tateishi and Masaru Yamaizumi and Haruo Ohmori and Cyrus Vaziri},
  journal={Molecular and cellular biology},
  year={2006},
  volume={26 9},
  pages={
          3527-40
        }
}
We have investigated mechanisms that recruit the translesion synthesis (TLS) DNA polymerase Polkappa to stalled replication forks. The DNA polymerase processivity factor PCNA is monoubiquitinated and interacts with Polkappa in cells treated with the bulky adduct-forming genotoxin benzo[a]pyrene dihydrodiol epoxide (BPDE). A monoubiquitination-defective mutant form of PCNA fails to interact with Polkappa. Small interfering RNA-mediated downregulation of the E3 ligase Rad18 inhibits BPDE-induced… CONTINUE READING
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