Rac binding to p67(phox). Structural basis for interactions of the Rac1 effector region and insert region with components of the respiratory burst oxidase.

@article{Nisimoto1997RacBT,
  title={Rac binding to p67(phox). Structural basis for interactions of the Rac1 effector region and insert region with components of the respiratory burst oxidase.},
  author={Yukio Nisimoto and Janine Freeman and S A Motalebi and Miriam Hirshberg and J. David Lambeth},
  journal={The Journal of biological chemistry},
  year={1997},
  volume={272 30},
  pages={18834-41}
}
Activation of the respiratory burst oxidase involves the assembly of the membrane-associated flavocytochrome b558 with the cytosolic components p47(phox), p67(phox), and the small GTPase Rac. Herein, the interaction between Rac and p67(phox) is explored using functional and physical methods. Mutually facilitated binding (EC50) of Rac1 and p67(phox) within the NADPH oxidase complex was demonstrated using steady state kinetic methods measuring NADPH-dependent superoxide generation. Direct binding… CONTINUE READING